A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones

Isolation of repetitive clones from human muscle cDNA library.

Human fetal muscle cDNA library was screened with a beta-myosin heavy chain gene fragment containing Alu sequences. Two cDNA clones AI and BII with 1.8 and 3 kb inserts respectively were chosen for further characterization by means of RNA and DNA hybridization procedures and sequencing. The clones appeared to contain repetitive sequences as well as single copy regions. They are actively transcr...

Isolation of MIDI cDNA clones

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ISOLATION AND EXPRESSION OF cDNA CLONES

Receptors for the Fc region of IgG (FcyR)' are expressed in a number of hematopoietic cell types and play important roles in several immunological processes such as phagocytosis of opsonized particulate antigens (1), clearance of immune complexes (2, 3), antibody-dependent cellular cytotoxicity (4, 5), signalling the production of inflammatory mediators (6), and regulation of Ig synthesis (7, 8...

cDNA Microarray Strategy for the Construction of the cDNA Array Preparation of Subtraction Libraries Prescreening of Library Clones cDNA Microarray Production cDNA Microarray Composition

Isolation of functional cDNA clones for human thymidylate synthase.

Thymidine auxotrophic mutants of mouse FM3A cells due to thymidylate synthase deficiency can be transformed into prototrophs by DNA-mediated gene transfer using total human DNA (Ayusawa, D., Shimizu, K., Koyama, H., Takeishi, K., and Seno, T. (1983) J. Biol. Chem. 258, 48-53). From one such transformed cell clone, cloned recombinant lambda phages containing DNA fragments were obtained recently ...